Yagisawa Laboratory - Publication

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Yagisawa F., Fujiwara T., Takemura T., Kobayashi Y., Sumiya N., Miyagishima S. Y., Nakamura S., Imoto Y., Misumi O., Tanaka K., Kuroiwa H., Kuroiwa T. ESCRT machinery mediates cytokinetic abscission in the unicellular red alga Cyanidioschyzon merolae. Front Cell Dev Biol. in press. 

Kuroiwa T., Ohnuma M., Imoto Y., Yagisawa F., Misumi O., Nagata N., Kuroiwa H. (2020) Evolutionary significance of the ring-like plastid nucleus in the primitive red alga Cyanidioschyzon merolae as revealed by drying. Protoplasma doi: 10.1007/s00709-020-01496-y.



Piña F., Yagisawa F., Obara K.,Gregerson J. D., Kihara A., Niwa M. (2018) Sphingolipids activate the endoplasmicreticulum stress surveillance pathway. J Cell Biol. 217: 495-505

Yoshida Y., Kuroiwa H., Shimada T., Yoshida M., Ohnuma M., Fujiwara T., Imoto Y., Yagisawa F., Nishida K., Hirooka S., Misumi O., Mogi Y., Akakabe Y., Matsushita K., Kuroiwa T. (2017) Glycosyltransferase MDR1 assembles a dividing ring for mitochondrial proliferation comprising polyglucan nanofilaments. Proc Natl Acad Sci U S A. 114: 13284-13289


Yagisawa F., Kuroiwa H., Fujiwara T.,Kuroiwa T. (2016) Intracellular structure of the unicellularred alga Cyanidioschyzon merolae in response to phosphate depletion and resupplementation. Cytologia 81: 341-347  

KuroiwaT., Ohnuma M., Imoto Y., Misumi O., Nagata N., Miyakawa I., Fujishima M.,Yagisawa F., Kuroiwa H. (2016) Genome size of the ultrasmall unicellular freshwater green alga, Medakamo hakoo 311, as determined by staining with 4′,6-Diamidino-2-phenylindole after microwave oven treatments: II. Comparison with Cyanidioschyzon merolae,Saccharomyces cerevisiae (n, 2n), and Chlorella variabilis. Cytologia 81: 69-76



Yagisawa F., Fujiwara T., Ohnuma M.,Kuroiwa H., Nishida K., Imoto Y., Yoshida Y., Kuroiwa T. (2013) Golgi inheritance in the primitive red alga, Cyanidioschyzon merolae. Protoplasma 250: 943-948


Imoto Y., Kuroiwa H., Yoshida Y., Ohnuma M., Fujiwara T., Yoshida M.,Nishida K., Yagisawa F., HirookaS., Miyagishima S. Y., Misumi O., Kawano S., Kuroiwa T. (2013) Single-membrane-bounded peroxisome division revealed by isolation of dynamin-based machinery. Proc Natl Acad Sci USA 110: 9583-9588  



Yagisawa F., Fujiwara T., Kuroiwa H.,Nishida K., Imoto Y., Kuroiwa T. (2012) Mitotic inheritance of endoplasmic reticulum in the primitive red alga Cyanidioschyzon merolae. Protoplasma 249:1129-1135


Yagisawa F. (2012) Mechanism of vacuole inheritance. Plant Morphology 24: 105-109



Imoto Y., Nishida K.,Yagisawa F., Yoshida Y., Ohnuma M., FujiwaraT., Kuroiwa H., Kawano S., Kuroiwa T. (2011) Involvement of ElongationFactor-1α in Cytokinesis without Actomyosin  Contractile Ring in the Primitive Red Alga Cyanidioschyzon merolae.Cytologia 76: 431-437


Imoto Y., Yoshida Y., Yagisawa F.,Kuroiwa H., Kuroiwa T. (2011) The cell cycle, including the mitotic cycle and organelle division cycles, as revealed by cytological observations. J Electron Microsc (Tokyo). 60 Suppl 1: S117-S136 



Yoshida Y., Kuroiwa H., Misumi, O.,Yoshida M., Ohnuma M., Fujiwara T., Yagisawa F., Hirooka S., Imoto Y.,Matsushita K. Kawano S. and Kuroiwa T. (2010) Chloroplasts divide by contraction of a bundle of nanofilaments consisting of polyglucan. Science 329:949-953


Fujiwara T., Kuroiwa H., Yagisawa F.,Ohnuma M., Yoshida Y., Yoshida M., Nishida K., Misumi O., Watanabe S., TanakaK., and Kuroiwa T. (2010) The coiled-coil protein VIG1 is essential for tethering vacuoles to mitochondria during vacuole inheritance of Cyanidioschyzon merolae. Plant Cell 22: 772-781.


Fujiwara T., Yagisawa F.,Ohnuma M.,  Misumi O., Yoshida Y.,Yoshida M., Nishida K., Kuroiwa H., Kuroiwa T. (2010) The vacuole binding to mitochondria by VIG1 contributes an equal inheritance of the vacuoles in Cyanidioschyzon merolae. Cytologia 75: 189-194



Hirooka S., Misumi O., Yoshida M., MoriT., Nishida K., Yagisawa F., Yoshida Y., Fujiwara T., Kuroiwa H. Kuroiwa T.(2009). Expression of the Cyanidioschyzon merolae stromal ascorbate peroxidase in Arabidopsis thaliana enhances thermotolerance. Plant Cell Rep 28: 1881-1893


Yagisawa F., Nishida K., Yoshida M.,Ohnuma M., Shimada T., Fujiwara T., Yoshida Y., Misumi O., Kuroiwa H., and Kuroiwa T. (2009) Identification of novel proteins in isolated polyphosphate vacuoles in the primitive red alga Cyanidioschyzon merolae. Plant J. 60:882-893


Fujiwara T., Misumi O., Tashiro K.,Yoshida Y., Nishida K., Yagisawa F., Imamura S., Yoshida M., Mori T., Tanaka K., Kuroiwa H., and Kuroiwa T. (2009) Periodic gene expression patterns during the highly synchronized cell nucleus and organelle division cycles in the unicellular red alga Cyanidioschyzon merolae. DNA Res. 16: 59-72



Kuroiwa T., Misumi O., Nishida K.,Yagisawa F., Yoshida Y., Fujiwara T., and Kuroiwa H. (2008) Vesicle,mitochondrial, and plastid division machineries with emphasis on dynamin and electron-dense rings. Int. Rev. Cell Mol. Biol. 271: 97-152



Yagisawa F., Nishida K., Kuroiwa H.,Nagata T., and Kuroiwa T. (2007) Identification and mitotic partitioning strategies of vacuoles in the unicellular red alga Cyanidioschyzon merolae.Planta 226:1017-1029


Nishida K., Yagisawa F., Kuroiwa H.,Yoshida Y., and Kuroiwa T. (2007) WD40 protein Mda1 is purified with Dnm1 and forms a dividing ring for mitochondria before Dnm1 in Cyanidioschyzon merolae. Proc. Natl. Acad. Sci. U S A 104: 4736-4741


Nozaki H., Takano H., Misumi O.,Terasawa K., Matsuzaki M., Maruyama S., Nishida K., Yagisawa F., Yoshida Y.,Fujiwara T., Takio S., Tamura K., Chung S. J., Nakamura S., Kuroiwa H., TanakaK., Sato N., and Kuroiwa T. (2007) A 100%-complete sequence reveals unusually simple genomic features in the hot-spring red alga Cyanidioschyzon merolae. BMC Biol. 5: 28



Yoshida Y., Kuroiwa H., Misumi O.,Nishida K., Yagisawa F., Fujiwara T., Nanamiya H., Kawamura F., and Kuroiwa T.(2006) Isolated chloroplast division machinery can actively constrict after stretching. Science 3131: 435-1438



Yagisawa F., Nishida K., Kuroiwa H.,Nagata T., and Kuroiwa T. (2005) Identification of lysosomes-like structures in a unicellular red alga Cyanidioschyzon merolae. Cytologia 70: 351-354


Nishida K., Yagisawa F., Kuroiwa H.,Nagata T., and Kuroiwa T. (2005) Cell cycle-regulated, microtubule-independent organelle division in Cyanidioschyzon merolae. Mol. Biol. Cell 52: 843-849


Misumi O., Matsuzaki M., Nozaki H.,Miyagishima S. Y., Mori T., Nishida K., Yagisawa F., Yoshida Y., Kuroiwa H., and Kuroiwa T. (2005) Cyanidioschyzon merolae genome. A Tool for facilitating comparable studies organelle biogenesis in photosynthetic eukaryotes. Plant Physiol. 137: 567-585



Yagisawa F., Nishida K., Okano, Y.,Minoda, A., Tanaka, K., and Kuroiwa, T. (2004) Isolation of cycloheximide-resistant mutants of Cyanidioschyzon merolae. Cytologia 69:97-100


Nishida K., Misumi O., Yagisawa F.,Kuroiwa H., Nagata T., and Kuroiwa T. (2004) Triple immunofluorescent labeling of FtsZ, dynamin, and EF-Tu reveals a loose association between the inner and outer membrane mitochondrial division machinery in the red alga Cyanidioschyzonmerolae. J. Histochem. Cytochem. 16:2493-2502


Minoda A., Sakagami R., Yagisawa F.,Kuroiwa T., and Tanaka K. (2004) Improvement of culture conditions and evidence for nuclear transformation by homologous recombination in a red alga,Cyanidioschyzon merolae 10D. Plant Cell Physiol. 45: 667-671


Matsuzaki M., Misumi O., Shin-I T.,Maruyama S., Takahara M., Miyagishima S. Y., Mori T., Nishida K., Yagisawa F.,Nishida K., Yoshida Y., Nishimura Y., Nakao S., Kobayashi T., Momoyama Y.,Higashiyama T., Minoda A., Sano M., Nomoto H., Oishi K., Hayashi H., Ohta F.,Nishizaka S., Haga S., Miura S., Morishita T., Kabeya Y., Terasawa K., SuzukiY., Ishii Y., Asakawa S., Takano H., Ohta N., Kuroiwa H., Tanaka K., Shimizu N., Sugano S., Sato N., Nozaki H., Ogasawara N., Kohara Y., and Kuroiwa T.(2004) Genome sequence of the ultra-small unicellular red alga Cyanidioschyzonmerolae 10D. Nature, 428: 653-657



Yagisawa F., Mori T., Higashiyama T.,Kuroiwa H., and Kuroiwa, T. (2003) Regulation of B. rapa chloroplast replication in vivo and in cultured leaf disks. Protoplasma 222: 139-148



Matsunaga S., Yagisawa F., Yamamoto, M.Uchida-Matsuda, W. Nakao, S. and Kawano S. (2002) LTR retrotransposons in the dioecious plant Silene latifolia. Genome 45: 745-751